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cell biology: an introduction to pathology / Abraham L. Kierszenbaum, Laura L. Tres. - Fourth edition. Philadelphia, PA: Elsevier/Saunders, - Studentconsult. Pathology Abraham L Kierszenbaum pdf histology and cell biology an introduction to pathology abraham l. kierszenbaum histology and cell biology an . Histology and Cell Biology - Abraham wildlifeprotection.info - Ebook download as PDF File .pdf) or read Team of Rivals: The Political Genius of Abraham Lincoln .

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View Test Prep - histology-and-cell-biology-an-introduction-to-pathology- wildlifeprotection.info from NURS at University of Pennsylvania. This book also is dedicated to the memory of my late colleague, Ernest N. Albert, Ph.D. He was a good friend and will be missed. Histology and Cell Biology 4th Edition PDF eBook Free Download. Histology and cell biology: an introduction to pathology / Abraham L. Kierszenbaum, Laura .

Thanks in advance for your time. Skip to content. Search for books, journals or webpages All Webpages Books Journals. An Introduction to Pathology. Abraham Kierszenbaum Laura Tres. Paperback ISBN: Published Date: During flagellar development in Chamydomo- present in photoreceptor cells. During IFT, precursors nas, cargo, raft, and motor proteins move bi-directionally for the assembly of the axoneme of a flagellum or a cilium between the plasma membrane and the axoneme.

Turnover proteins move in reverse, from the keratin-like outer dense fiber proteins occupy the space axoneme tip to the cytoplasm, with the help of another between the axoneme and the plasma membrane. Assembly blocks fibrous sheath space rather than along the axoneme- are passengers, or cargo, carried on a protein raft con- plasma membrane passageway as in Chlamydomonas.

Examples are the release across the finding of the raft protein Polaris in the manchette nuclear pore complexes into the cytoplasm of somatic Taulman et al. What makes Ran- because the Tg mutant mouse displays abnormal GTPase interesting, in addition to its known role as a development of the photoreceptor outer segment and regulator of nucleocytoplasmic transport, is its reloca- degeneration of the retina Pazour et al.

An intriguing ques- displaying a manchette but an abortive flagellum un- tion is how can several different proteins be mobilized by published observation. These findings suggest that IMT at a precise developmental time? Although there protein raft, which consists of several components with are still myriad unanswered questions, IMT emerges as protein-protein interaction properties, it is possible to a compelling mechanism by which the manchette can envision an efficient way of mobilizing cargos simulta- deliver building blocks for assembling the sperm tail, neously to specific sites during sperm development.

The molecular motors kinesin Miller et al.

Blatch GL, Lassle M. The tetratricopeptide repeat: a structural motif mediating protein-protein interactions.

Bioessays — KHC structure and function during the eukaryotic cell division cycle? Kinesin light chain KLC3 expression in testis is restricted to spermatids. KLC3 is expressed by round and Biol Reprod — Spermatid manchette: plugging proteins to tails, thus suggesting a unique function Junco et al.

Mol Reprod Dev — Ran, a GTP- well as six tetratricopepide repeat TPR motifs involved binding protein involved in nucleocytoplasmic transport and micro- tubule nucleation, relocates from the manchette to the centrosome in protein-protein interaction Blatch and Lassle, Therefore, the molecular pre-conditions are Goldstein LSB.

Genetic evidence for selective transport of available for KLC to interact via TPR motifs with similar opsin and arrestin by kinesin-II in mammalian photoreceptors. Cell — What happens Meistrich ML. The association of K5 with microtubules of the manchette was reported previously Tres and Kierszenbaum, During step 17, when the manchette has fully disassembled, K5 immunoreactive sites occupy a caudal nuclear distribution Figure 3, H—K.

A strong propidium iodide nuclear-stained region correlates with the cap-like projection of the acrosome Figure 3N.

Figure 3. A—C A step 8 spermatid displays a top view of the acrosome acr, A. D and E Step 8 spermatid subjected to hypotonic treatment to remove the cytoplasm and stained with anti-K5 serum and propidium iodide PI. The K5-immunoreacted acroplaxome arrow remains attached to the spermatid nucleus.

F and G Step 12 spermatid displaying the K5 immunoreactive manchette crossed arrow and acroplaxome arrow attached to the elongating PI-stained spermatid nucleus. H and I Step 17 spermatid. The K5 stained component of the acroplaxome occupies a caudal site arrow , and the spermatid tail arrowhead displays similar immunoreactivity.

J and K Step 18 spermatid showing a more caudal localization of K5 immunoreactivity. An immunoreactive spermatid tail arrowhead is seen in the field.


L—N Step 8 spermatid is devoid of cytoplasm after hypotonic treatment but retains the acrosome acr, arrow. N Strong and uniform cap-like PI nuclear staining at the acroplaxome site denoted by arrowheads. O and P Step 12 spermatid. The manchette crossed arrow in P seems essentially nonimmunoreactive by immunofluorescence compared with F and G.

Figure 4. The attachment of the rat spermatid acroplaxome to the nuclear envelope is not disrupted by Triton X extraction before fixation. A—D A step 4 to 5 spermatid displays in A its acrosome arrow lodged in a shallow depression of the nucleus crossed arrow.

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The Golgi apparatus arrowhead remains attached to the acrosome. The K5-immunoreactive acroplaxome is shown in B.

C A linear profile of propidium iodide-stained nuclear material is seen along the shallow depression crossed arrow.

E—H Step 6 spermatid displays the same features observed in the A—D sequence except that the linear propidium iodide stained nuclear material is more extensive and matching the length of the acroplaxome.

I—L Step 6 spermatid showing a frontal view of the acrosome arrow surrounded by a dense edge crossed arrow and associated Golgi apparatus arrowhead. In contrast, the insets in panels J and K show the marginal ring of the K5-stained acroplaxome inset in J overlapping the 4,6-diamidinophenylindole-stained nuclei inset in K not visualized after PNA-Alexa Fluor staining. Figure 4, A—D steps 4 and 5 of spermiogenesis , and E—H step 6 demonstrate that a combination of sucrose hypotonic treatment and a brief Triton X extraction step before fixation removes most of spermatid cytoplasm, leaving relatively undisturbed the acrosomal vesicle and associated Golgi apparatus.

A shallow nuclear indentation accommodates the acrosome. The boundary of shallow indentation displays a distinct propidium iodide-stained nuclear band Figure 4G , suggesting a different degree of chromatin condensation at this site. Figure 4, I—L , demonstrates that the entire acrosome, including the marginal region of a step 6 spermatid, stains evenly, in contrast with the ring-like distribution of K5 seen in the same developmental step Figure 4, J and K , inset.

The structure of the ring was analyzed further by electron microscopy. Parallel aligned and obliquely oriented bundles of filaments with respect the nucleus can be visualized at the leading edges of the acroplaxome rat spermatid, Figure 5A. In a sagittal view mouse spermatid; Figure 5B , the marginal ring of the acroplaxome displays cross-sectioned nm-thick filaments attached to a dense plaque bound to the inner acrosome membrane.

F-Actin microfilaments 7 nm in thickness in the Sertoli cell ectoplasmic region and microtubules of the manchette 25 nm in width in the same microscopic field provide support to the different thickness of the intermediate filaments.

The K5 immunoreactive filaments can also be visualized by immunofluorescence at the expected position and angular orientation Figure 5, C and D. Figure 5. A Step 9 rat spermatid sectioned at oblique angle demonstrates the central portion of the acroplaxome bracket flanked by the marginal ring in which intermediate filaments bundles are visualized boxes. The manchette arrow and adjacent Sertoli cell ectoplasmic F-actin bundles arrowheads are seen.

B Sagittal view of the acroplaxome marginal ring of a step 10 mouse spermatid. The rectangular box denotes a bundle of intermediate filaments attached to a dense plaque associated with the inner acrosome membrane.

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The thickness of the intermediate filaments 10 nm can be compared with adjacent microfilaments 7 nm, circle of the ectoplasmic Sertoli cell region where actin bundles are located black arrowheads and microtubules of the manchette 25 nm, arrow inserted in the perinuclear ring white arrowhead. The asterisk indicates a groove separating the acroplaxome-acrosome leading edge from the perinuclear ring of the manchette. C and D Step 12 rat spermatid immunoreacted with anti-K5 affinity-purified serum showing the staining of the acroplaxome, the manchette crossed arrow , and spermatid tail arrow.

The parallel lines in D indicate the angular orientation of the intermediate filaments of the acroplaxome margin. Immunogold electron microscopy demonstrates the presence of K5 immunoreactivity in the subacrosomal region where the acroplaxome and marginal ring are located Figure 6, A and B.


Figure 7 presents a summary of the relevant aspects of acroplaxome development in rat spermatids within a graphic and descriptive context. Figure 6. A Gold particles representing K5 antigenic sites are seen along the rat spermatid acroplaxome indicated by the brackets shown in an tangential orientation.

B K5 immunoreactive sites at the rat spermatid acroplaxome margin rectangular box and its adjacent region bracket. Figure 7. A Step 3.

A Golgi-derived G acrosome vesicle Acr contacts the nuclear envelope but the acroplaxome is not visualized. B Step 4. The acrosome vesicle is lodged in a shallow depression of the nucleus arrows where the density of the acroplaxome is seen.

C Step 5. The inner acrosomal membrane-associated plaque dashed box at the leading edge of the acroplaxome arrow is detected. D Step 6. An intermediate filament bundle arrowheads is seen attached to the acrosome Acr -linked plaque. The density of the acroplaxome is indicated by the arrows. E Step 7. Shallow indentations at the margins of the acroplaxome dashed boxes are detected when the acrosome Acr initiates its caudal descent.

Neither the groove nor the manchette are present. The double-crossed arrow points to the assembling head-to-tail coupling apparatus. F Step 9.

Elongation of the spermatid nucleus occurs as the marginal regions of the acrosome and acroplaxome dashed boxes continue their descent. The manchette Man is now assembled. G Step H Step The leading marginal portion of the acroplaxome displays intense K5 immunoreactivity dashed box.

In the bar graph, the white boxes indicate the average diameter micrometers of the acroplaxome, and the gray boxes indicate the width micrometers of the acroplaxome marginal ring. In the illustrations representing rat spermiogenic steps, the gray region indicates the Golgi apparatus, the white space corresponds to the acrosome vesicle and acrosome, and the red line denotes the position of the acroplaxome.

The manchette is indicated by two green lines extending into the cytoplasm. In step 18, the centrosome region continuous with a long red line representing the developing tail displays K5 immunoreactivity. The Acroplaxome and Manchette in Abnormally Shaped Spermatid Nuclei of the azh Mutant Mouse Mutant mouse models in which spermatid nuclear shaping is defective can provide clues concerning the significance of the acroplaxome in the elongation of the spermatid nucleus.

Previous studies have shown that, in the azh mutant mouse, a relative number of spermatids display abnormally shaped nuclei, sperm have coiled tails Mochida et al. Figure 8A illustrates an elongating spermatid in which the acroplaxome-containing region is particularly indented. A similar feature was observed in mouse offspring produced from normal mouse oocytes injected with sperm heads from the azh mutant see Figure 4, B and C , in Akutsu et al. An additional deformity during the nuclear shaping of the azh spermatid mutant is the presence of a nuclear constriction at the site where the perinuclear ring of the manchette is located Figure 8B.

Figure 8. The acroplaxome and perinuclear ring of the manchette in azh mutant mouse spermatids.

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A The acroplaxome corresponds to the top folded portion of the elongating spermatid nucleus capped by the acrosomal sac Acr. The boxes indicate the marginal ring; the crossed arrow indicates a deep infolding of the acroplaxome.

The arrows point to actin filament bundles and associated endoplasmic reticulum cisternae in the Sertoli cell ectoplasm. The microtubules of the manchette are present.

B The apical pole of the condensing and elongating nucleus of a spermatid is covered by the Acr.Key words: manchette, acrosome, acroplaxome, sperm head shaping Introduction Spermatids are highly polarized cells. The K5 stained component of the acroplaxome occupies a caudal site arrow , and the spermatid tail arrowhead displays similar immunoreactivity. What makes Ran- because the Tg mutant mouse displays abnormal GTPase interesting, in addition to its known role as a development of the photoreceptor outer segment and regulator of nucleocytoplasmic transport, is its reloca- degeneration of the retina Pazour et al.

Opposite to the intermediate filament—plaque complex is another thin plaque spanning across a shallow indentation in the spermatid nuclear envelope and linked to a nuclear lamina see Figure 9 for a summary diagram.

Upon disassembly of the manchette, Ran development.

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