BINH PHAP TON TU PDF
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Batch Image Converter. Be sure to keep a log of which spot contains which sample!
Multiple spotting will be required for the Diet Coca-Cola samples about times. Handle it carefully by the edges and do not bend. Two Diet Coke samples.
You may want to number the spots to number the spots to keep them straight. When the solvent is about 1 cm from the top. Allow the protein to dry for a few minutes. Remove the plates from the oven. Make sure you keep track of which spot is which and allow the spots to dry. You will use these samples for spotting the plate.
Place the spotted TLC plate in the developing tank you prepared in step 3. Allow the spot to dry each time before reapplying in order to prevent the spotting area from getting too large.
In this experiment you will determine the Rf values of three pure substances and compare these data with the results observed from diet pop samples in order to determine which of the substances are present in the diet pop.
Lightly mark 5 positions where your samples will be spotted. Allow the solvent to evaporate off of the plate in the fume hood!
The drying process can be accelerated by using an air gun. Add acid until no further precipitation occurs.
Using gloves. Weigh and record the mass of casein from the milk sample. Give the structures of phenylalanine and aspartic acid. Measure and calculate the Rf values of the two spots A and B shown in the hypothetical TLC plate shown on page 2 of the introduction. Show the calculations below. It should be done to scale and not only show the positions of the spot but their sizes and relative darkness as well.
TLC Observations: Explain using a chemical equation. Why does the milk protein casein precipitate when acetic acid is added? Use Rf values to support your conclusions. Which were found in the aged Diet Coke. Are these results what should have been expected? Compare the experimental value you obtained for the percentage of casein in milk to a literature value and discuss.
Which of the three reference compounds phenylalanine. Flag for inappropriate content.
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Madalina Irina Agafitei. Muhamad Saiful.Peptide linkages hold amino acids together. Lightly mark 5 positions where your samples will be spotted. Even when face to face with an enemy, surprise can still be employed by attacking where he least expects it. Try to let the spots only spread out to about 1 mm in diameter. Taufiq Ali.
In fact, different pH conditions can lead to different forms of the acid.